In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
The Escherichia coli and vaccinia virus-based reverse genetics strategies have been broadly utilized for the manipulation and engineering of coronavirus genomes. These strategies, nonetheless, present numerous limitations and are usually robust to find out in a effectively timed methodology for (re-)rising viruses. On this chapter, we present a model new frequent reverse genetics platform for the assembly and engineering of infectious full-length cDNAs using yeast-based transformation-associated recombination cloning.
This novel assembly approach not solely ends in safe coronavirus infectious full-length cDNAs cloned throughout the yeast Saccharomyces cerevisiae however moreover fosters and accelerates the manipulation of their genomes.
Such a platform is broadly related for the scientific neighborhood, as a result of it requires no explicit gear and may very well be carried out in an abnormal laboratory setting. The protocol described may very well be merely tailor-made to only about all acknowledged or rising coronaviruses, paying homage to Heart East respiratory syndrome coronavirus (MERS-CoV).

pfizeracademy
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C1534035 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534089 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534090 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534105 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534106 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534118 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534122 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534142 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534149 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534152 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534160 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534169 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534183 | Biochain | 40 reactions | EUR 939 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534188 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534189 | Biochain | 40 reactions | EUR 1051 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534190 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534194 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534200 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534201 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534206 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534218 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534246 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534248 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534260 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534263 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534264 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534265 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534267 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534268 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534273 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534274 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534283 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534091 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534092 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534095 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534096 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534100 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534115 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534133 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534161 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534171 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534226 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534234 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534250 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534251 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534252 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534253 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534275 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534276 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534278 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534013 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534020 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534101 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534150 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534151 | Biochain | 40 reactions | EUR 376 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534227 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534230 | Biochain | 40 reactions | EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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RC326-12 | BBI Biotech | 2ug | EUR 104.38 |
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4-PAA033Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa) |
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4-PAA546Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA) |
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4-PAA979Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA) |
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4-MAA080Si21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8) |
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4-MAA080Si22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8) |
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4-PAA078Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5) |
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4-PAA079Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6) |
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4-PAA080Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8) |
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4-MAA267Si21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK) |
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EMKC0011 | Abclonal | 96Tests | EUR 521 |
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AMM02279G | Leading Biology | 0.05ml | EUR 484 |
Description: A Monoclonal antibody against Human CD4 (clone 5D9). The antibodies are raised in Mouse and are from clone 5D9. This antibody is applicable in WB and IHC-P |
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4-PAA033Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with APC. |
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4-PAA033Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with Biotin. |
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4-PAA033Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with Cy3. |
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4-PAA033Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with FITC. |
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4-PAA033Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with HRP. |
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4-PAA033Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with PE. |
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4-PAA190Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c) |
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4-PAA546Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with APC. |
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4-PAA546Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with Biotin. |
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4-PAA546Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with Cy3. |
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4-PAA546Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with FITC. |
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4-PAA546Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with HRP. |
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4-PAA546Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with PE. |
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4-PAA979Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with APC. |
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4-PAA979Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with Biotin. |
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4-PAA979Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with Cy3. |
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4-PAA979Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with FITC. |
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4-PAA979Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with HRP. |
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4-PAA979Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with PE. |
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4-PAB409Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB) |
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4-MAA782Si22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2) |
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4-MAA079Si22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Rhesus monkey Interleukin 6 (IL6) |
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4-MAA080Si21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC. |
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4-MAA080Si21-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Biotin. |
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4-MAA080Si21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3. |
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4-MAA080Si21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC. |
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4-MAA080Si21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP. |
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4-MAA080Si21-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE. |
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4-MAA080Si22-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC. |
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4-MAA080Si22-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Biotin. |
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4-MAA080Si22-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3. |
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4-MAA080Si22-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC. |
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4-MAA080Si22-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP. |
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4-MAA080Si22-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE. |
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4-PAA078Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with APC. |
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4-PAA078Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with Biotin. |
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4-PAA078Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with Cy3. |
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4-PAA078Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with FITC. |
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4-PAA078Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with HRP. |
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4-PAA078Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with PE. |
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4-PAA079Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with APC. |
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4-PAA079Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with Biotin. |
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4-PAA079Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with Cy3. |
![]() |
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4-PAA079Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with FITC. |
![]() |
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4-PAA079Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with HRP. |
![]() |
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4-PAA079Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with PE. |
![]() |
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4-PAA080Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC. |
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4-PAA080Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Biotin. |
![]() |
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4-PAA080Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3. |
![]() |
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4-PAA080Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC. |
![]() |
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4-PAA080Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP. |
![]() |
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4-PAA080Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE. |
![]() |
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4-MAA267Si21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with APC. |
![]() |
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4-MAA267Si21-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with Biotin. |
![]() |
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4-MAA267Si21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with Cy3. |
![]() |
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4-MAA267Si21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with FITC. |
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4-MAA267Si21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with HRP. |
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4-MAA267Si21-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with PE. |
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D1534999-G01 | Biochain | 100 ug | EUR 188 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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D1534999-G02 | Biochain | 100 ug | EUR 188 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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D6534149 | Biochain | 5 ug | Ask for price |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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FNSA-0092 | FN Test | 500 uL | EUR 306.6 |
Description: Rabbit anti Rhesus Monkey IgG secondary antibody |
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FNSA-0093 | FN Test | 500 uL | EUR 306.6 |
Description: Rabbit anti Rhesus Monkey IgM secondary antibody |
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RMS05-0050 | Equitech | 50 ml | EUR 741 |
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RMS05-0100 | Equitech | 100 ml | EUR 1140.1 |
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RMS05-0500 | Equitech | 500 ml | Ask for price |
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APR15351G | Leading Biology | 0.1ml | EUR 484 |
Description: A Monoclonal antibody against Human CD4. The antibodies are raised in Mouse and are from clone B486A1. This antibody is applicable in FC, E |
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DLR-CD4-Hu-48T | DL Develop | 48T | EUR 498 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Cluster Of Differentiation 4 (CD4) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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DLR-CD4-Hu-96T | DL Develop | 96T | EUR 647 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Cluster Of Differentiation 4 (CD4) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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DLR-CD4-Mu-48T | DL Develop | 48T | EUR 508 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation 4 (CD4) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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DLR-CD4-Mu-96T | DL Develop | 96T | EUR 661 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation 4 (CD4) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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DLR-CD4-Ra-48T | DL Develop | 48T | EUR 528 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation 4 (CD4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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DLR-CD4-Ra-96T | DL Develop | 96T | EUR 690 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation 4 (CD4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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RDR-CD4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 522 |
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RDR-CD4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 724 |
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RDR-CD4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
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RDR-CD4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
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RDR-CD4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 558 |
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RDR-CD4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 776 |
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RD-CD4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
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RD-CD4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
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RD-CD4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
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RD-CD4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
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RD-CD4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
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RD-CD4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
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VAng-1300Lsx-100g | Creative Biolabs | 100 µg | EUR 738 |
Description: Rhesus macaque CD4, His tag, is expressed in HEK 293 cells. (Uniprot ID: G7N5T8) |
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VAng-1300Lsx-1mg | Creative Biolabs | 1 mg | EUR 4449 |
Description: Rhesus macaque CD4, His tag, is expressed in HEK 293 cells. (Uniprot ID: G7N5T8) |
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4-PAA033Si01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with APC-Cy7. |
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4-PAA133Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Tumor Necrosis Factor Alpha (TNFa) |
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4-PAA190Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with APC. |
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4-PAA190Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with Biotin. |
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4-PAA190Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with Cy3. |
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4-PAA190Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with FITC. |
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4-PAA190Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with HRP. |
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4-PAA190Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c). This antibody is labeled with PE. |
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4-PAA546Si01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with APC-Cy7. |
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4-PAA223Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 1 Receptor Antagonist (IL1RA) |
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4-PAA087Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Monocyte Chemotactic Protein 1 (MCP1) |
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4-PAB028Si01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat, Rhesus monkey, Bovine Albumin (ALB) |
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4-PAA979Si01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with APC-Cy7. |
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4-PAB409Si01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with APC. |
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4-PAB409Si01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with Biotin. |
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4-PAB409Si01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with Cy3. |
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4-PAB409Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with FITC. |
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4-PAB409Si01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with HRP. |
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4-PAB409Si01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB). This antibody is labeled with PE. |
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4-MAA782Si22-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with APC. |
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4-MAA782Si22-Biotin | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with Biotin. |
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4-MAA782Si22-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with Cy3. |
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4-MAA782Si22-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with FITC. |
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4-MAA782Si22-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with HRP. |
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4-MAA782Si22-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2). This antibody is labeled with PE. |
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4-MAA079Si22-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with APC. |
Synthesis of Full-Dimension cDNA Infectious Clones of Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid throughout the Silencing Suppressor Hc-Skilled
- Soybean mosaic virus (SMV), which belongs to the Potyviridae, causes very important reductions in soybean yield and seed prime quality. On this look at, every tag-free and reporter gene inexperienced fluorescent protein (GFP)-containing infectious clones for the SMV N1 strain have been constructed by Gibson assembly and with the yeast homologous recombination system, respectively.
- Every infectious clones are acceptable for agroinfiltration on the model host benthamiana and current strong infectivity for the pure host soybean and several other different totally different legume species. Every infectious clones have been seed transmitted and prompted typical virus indicators on seeds and progeny crops. We used the SMV-GFP infectious clone to further look at the perform of key amino acids throughout the silencing suppressor helper component-proteinase (Hc-Skilled).
- Amongst twelve amino acid substitution mutants, the co-expression of mutant 2-with an Asparagine→Leucine substitution at place 182 of the FRNK (Phe-Arg-Asn-Lys) motif-attenuated viral indicators and alleviated the host progress retardation introduced on by SMV.
- Moreover, the Hc-Prom2 mutant confirmed stronger oligomerization than wild-type Hc-Skilled. Taken collectively, the SMV infectious clones may be useful for analysis of host-SMV interactions and helpful gene characterization in soybeans and related legume species, notably by means of seed transmission properties. Furthermore, the SMV-GFP infectious clone will even facilitate helpful analysis of every virus and host genes in an benthamiana transient expression system.
Profiling of rice Cd-tolerant genes by means of yeast-based cDNA library survival screening
The bioaccumulation of cadmium (Cd) in crop and the following meals chain has aroused intensive issues. Nonetheless, the underlying molecular mechanisms of plant Cd tolerance keep to be clarified from the viewpoint of novel candidate genes.
Proper right here we described a extraordinarily atmosphere pleasant technique for preliminary determining rice Cd-tolerant genes by means of the yeast-based cDNA library survival screening blended with high-throughput sequencing approach. About 690 gene isoforms have been acknowledged as being Cd-tolerant candidates using this shotgun technique.
Among the many many Cd-tolerant genes acknowledged, numerous lessons of genes paying homage to BAX inhibitor (BI), NAC transcription parts and Quick ALkalinization Parts (RALFs) have been of particular curiosity, and their carry out of Cd tolerance was further validated via heterologous expression, which urged that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves.
Referring to the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated space (HMAD) isoprenylated protein HIPP42 was considerably noteworthy. Further elucidation of these genes associated to Cd tolerance in rice will revenue agricultural actions.
Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Period for scRNA-Seq
Single-cell RNA-sequencing (scRNA-seq) permits an entire analysis of the transcriptome of explicit particular person cells by next-generation sequencing. ScRNA-seq provides an unbiased technique to investigate the cellular heterogeneity and dynamics of varied natural strategies, along with the immune system. Optimization of the technical procedures carried out earlier to RNA-seq analysis is essential to the success of a scRNA-seq experiment.
Proper right here, three essential experimental procedures are described: (1) the isolation of immune CD8a+ T cells from essential murine tissue, (2) the expertise of single-cell cDNA libraries using the 10× Genomics Chromium Controller and the Chromium Single Cell 3′ Reply, and (3) cDNA library prime quality administration. On this protocol, CD8a+ T cells are isolated from murine spleen tissue, nonetheless any cell form of curiosity may very well be enriched and used for single-cell cDNA library expertise and subsequent RNA-seq experiments.
![]() Human Chemokine C-C-Motif Receptor 6 (CCR6) ELISA Kit |
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RD-CCR6-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 723 |
![]() Anti-CCR6/CCR6 Antibody |
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PA1201 | BosterBio | 100ug/vial | EUR 294 |
![]() CCR6 |
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MO15087 | Neuromics | 100 ug | EUR 409 |
![]() CCR6 antibody |
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70R-13797 | Fitzgerald | 100 ug | EUR 322 |
Description: Affinity purified Rabbit polyclonal CCR6 antibody |
![]() CCR6 Antibody |
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37466-100ul | SAB | 100ul | EUR 252 |
![]() CCR6 antibody |
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10R-1071 | Fitzgerald | 100 ul | EUR 316 |
Description: Mouse monoclonal CCR6 antibody |
![]() CCR6 Antibody |
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1-CSB-PA004845EA01HU | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000 |
![]() CCR6 Antibody |
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1-CSB-PA943255 | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:50-1:200 |
![]() CCR6 Antibody |
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DF10207 | Affbiotech | 200ul | EUR 304 |
Description: CCR6 Antibody detects endogenous levels of total CCR6. |
![]() CCR6 Antibody |
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1-CSB-PA244343 | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200 |
![]() CCR6 siRNA |
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20-abx910877 | Abbexa |
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![]() CCR6 siRNA |
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20-abx910878 | Abbexa |
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![]() CCR6 Antibody |
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ABD10207 | Lifescience Market | 100 ug | EUR 438 |
![]() CCR6 Rabbit pAb |
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A16206-100ul | Abclonal | 100 ul | EUR 308 |
![]() CCR6 Rabbit pAb |
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A16206-200ul | Abclonal | 200 ul | EUR 459 |
![]() CCR6 Rabbit pAb |
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A16206-20ul | Abclonal | 20 ul | EUR 183 |
![]() CCR6 Rabbit pAb |
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A16206-50ul | Abclonal | 50 ul | EUR 223 |
![]() CCR6 Blocking Peptide |
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DF10207-BP | Affbiotech | 1mg | EUR 195 |
![]() Anti-CCR6 Antibody |
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A00957 | BosterBio | 100ug/vial | EUR 334 |
![]() Anti-CCR6 Antibody |
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A00061-1 | BosterBio | 200ug | EUR 397 |
Description: Goat Polyclonal CCR6 Antibody. Validated in ELISA, IHC and tested in Human, Mouse. |
![]() CCR6 Blocking Peptide |
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20-abx061923 | Abbexa |
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![]() CCR6 Conjugated Antibody |
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C37466 | SAB | 100ul | EUR 397 |
![]() CCR6 cloning plasmid |
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CSB-CL004845HU-10ug | Cusabio | 10ug | EUR 233 |
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Description: A cloning plasmid for the CCR6 gene. |
![]() CCR6 Polyclonal Antibody |
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A50105 | EpiGentek | 100 µg | EUR 570.55 |
Description: kits suitable for this type of research |
![]() CCR6 inhibitor 1 |
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HY-112701 | MedChemExpress | 10mg | EUR 911 |
![]() Anti-CCR6 antibody |
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STJ118659 | St John's Laboratory | 100 µl | EUR 277 |
![]() Anti-CCR6 Antibody |
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STJ500414 | St John's Laboratory | 100 µg | EUR 476 |
![]() CCR6 Antibody, HRP conjugated |
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1-CSB-PA004845EB01HU | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
![]() CCR6 Antibody, FITC conjugated |
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1-CSB-PA004845EC01HU | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
![]() CCR6 Antibody, Biotin conjugated |
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1-CSB-PA004845ED01HU | Cusabio |
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Description: A polyclonal antibody against CCR6. Recognizes CCR6 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
![]() Human CCR6 ELISA Kit |
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ELA-E2014h | Lifescience Market | 96 Tests | EUR 824 |
![]() CCR6 ELISA KIT|Human |
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EF006138 | Lifescience Market | 96 Tests | EUR 689 |
![]() Mouse CCR6 shRNA Plasmid |
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20-abx969534 | Abbexa |
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![]() Human CCR6 shRNA Plasmid |
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20-abx950871 | Abbexa |
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![]() CCR6 Recombinant Protein (Human) |
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RP006232 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Rat) |
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RP193790 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122255 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122258 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122261 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122264 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122267 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122270 | ABM | 100 ug | Ask for price |
![]() CCR6 Recombinant Protein (Mouse) |
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RP122273 | ABM | 100 ug | Ask for price |
![]() Anti-CCR6 Antibody (Biotin) |
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STJ500416 | St John's Laboratory | 100 µg |